[meteorite-list] Holographic Imaging Could Be Used to Detect Signs of Life in Space

Ron Baalke baalke at zagami.jpl.nasa.gov
Thu Aug 24 19:39:27 EDT 2017


Holographic Imaging Could Be Used to Detect Signs of Life in Space

Engineers explore ways to sample and identify living microbes in the outer 
solar system

July 20, 2017

We may be capable of finding microbes in space - but if we did, could 
we tell what they were, and that they were alive?

This month the journal Astrobiology is publishing a special issue dedicated 
to the search for signs of life on Saturn's icy moon Enceladus. Included 
is a paper from Caltech's Jay Nadeau and colleagues offering evidence 
that a technique called digital holographic microscopy, which uses lasers 
to record 3-D images, may be our best bet for spotting extraterrestrial 

No probe since NASA's Viking program in the late 1970s has explicitly 
searched for extraterrestrial life - that is, for actual living organisms. 
Rather, the focus has been on finding water. Enceladus has a lot of water - an 
ocean's worth, hidden beneath an icy shell that coats the entire surface. 
But even if life does exist there in some microbial fashion, the difficulty 
for scientists on Earth is identifying those microbes from 790 million 
miles away.

"It's harder to distinguish between a microbe and a speck of dust than 
you'd think," says Nadeau, research professor of medical engineering and 
aerospace in the Division of Engineering and Applied Science. "You have 
to differentiate between Brownian motion, which is the random motion of 
matter, and the intentional, self-directed motion of a living organism."

Enceladus is the sixth-largest moon of Saturn, and is 100,000 times less 
massive than Earth. As such, Enceladus has an escape velocity - the minimum 
speed needed for an object on the moon to escape its surface - of just 
239 meters per second. That is a fraction of Earth's, which is a little 
over 11,000 meters per second.

Enceladus's minuscule escape velocity allows for an unusual phenomenon: 
enormous geysers, venting water vapor through cracks in the moon's icy 
shell, regularly jet out into space. When the Saturn probe Cassini flew 
by Enceladus in 2005, it spotted water vapor plumes in the south polar 
region blasting icy particles at nearly 2,000 kilometers per hour to an 
altitude of nearly 500 kilometers above the surface. Scientists calculated 
that as much as 250 kilograms of water vapor were released every second 
in each plume. Since those first observations, more than a hundred geysers 
have been spotted. This water is thought to replenish Saturn's diaphanous 
E ring, which would otherwise dissipate quickly, and was the subject of 
a recent announcement by NASA describing Enceladus as an "ocean world" 
that is the closest NASA has come to finding a place with the necessary 
ingredients for habitability.

Water blasting out into space offers a rare opportunity, says Nadeau. 
While landing on a foreign body is difficult and costly, a cheaper and 
easier option might be to send a probe to Enceladus and pass it through 
the jets, where it would collect water samples that could possibly contain 

Assuming a probe were to do so, it would open up a few questions for engineers 
like Nadeau, who studies microbes in extreme environments. Could microbes 
survive a journey in one of those jets? If so, how could a probe collect 
samples without destroying those microbes? And if samples are collected, 
how could they be identified as living cells?

The problem with searching for microbes in a sample of water is that they 
can be difficult to identify. "The hardest thing about bacteria is that 
they just don't have a lot of cellular features," Nadeau says. Bacteria 
are usually blob-shaped and always tiny - smaller in diameter than a strand 
of hair. "Sometimes telling the difference between them and sand grains 
is very difficult," Nadeau says.

Some strategies for demonstrating that a microscopic speck is actually 
a living microbe involve searching for patterns in its structure or studying 
its specific chemical composition. While these methods are useful, they 
should be used in conjunction with direct observations of potential microbes, 
Nadeau says.

"Looking at patterns and chemistry is useful, but I think we need to take 
a step back and look for more general characteristics of living things, 
like the presence of motion. That is, if you see an E. coli, you know 
that it is alive - and not, say, a grain of sand - because of the way 
it is moving," she says. In earlier work, Nadeau suggested that the movement 
exhibited by many living organisms could potentially be used as a robust, 
chemistry-independent biosignature for extraterrestrial life. The motion 
of living organisms can also be triggered or enhanced by "feeding" the 
microbes electrons and watching them grow more active.

To study the motion of potential microbes from Enceladus's plumes, Nadeau 
proposes using an instrument called a digital holographic microscope that 
has been modified specifically for astrobiology.

In digital holographic microscopy, an object is illuminated with a laser 
and the light that bounces off the object and back to a detector is measured. 
This scattered light contains information about the amplitude (the intensity) 
of the scattered light, and about its phase (a separate property that 
can be used to tell how far the light traveled after it scattered). With 
the two types of information, a computer can reconstruct a 3-D image of 
the object—one that can show motion through all three dimensions.

"Digital holographic microscopy allows you to see and track even the tiniest 
of motions," Nadeau says. Furthermore, by tagging potential microbes with 
fluorescent dyes that bind to broad classes of molecules that are likely 
to be indicators of life - proteins, sugars, lipids, and nucleic acids - you 
can tell what the microbes are made of," she says.

To study the technology's potential utility for analyzing extraterrestrial 
samples, Nadeau and her colleagues obtained samples of frigid water from 
the Arctic, which is sparsely populated with bacteria; those that are 
present are rendered sluggish by the cold temperatures.

With holographic microscopy, Nadeau was able to identify organisms with 
population densities of just 1,000 cells per milliliter of volume, similar 
to what exists in some of the most extreme environments on Earth, such 
as subglacial lakes.  For comparison, the open ocean contains about 10,000 
cells per milliliter and a typical pond might have 1-10 million cells 
per milliliter. That low threshold for detection, coupled with the system's 
ability to test a lot of samples quickly (at a rate of about one milliliter 
per hour) and its few moving parts, makes it ideal for astrobiology, Nadeau 

Next, the team will attempt to replicate their results using samples from 
other microbe-poor regions on Earth, such as Antarctica.

Nadeau collaborated with Caltech graduate student Manuel Bedrossian and 
Chris Lindensmith of JPL.

Written by Robert Perkins

Robert Perkins
(626) 395-1862
rperkins at caltech.edu

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